Journal: International Journal of Molecular Sciences

Article Title: Trichinella spiralis Paramyosin Alleviates Collagen-Induced Arthritis in Mice by Modulating CD4 + T Cell Differentiation

doi: 10.3390/ijms25126706

Figure Lengend Snippet: Administration of r Ts -Pmy ameliorated the immune imbalance of CD4 + T cells in CIA mice paw joints. ( A ) The concentration of cytokines in paws of mice in each group; ( B ) number of CD4 + T cells in paws, scale bar: 50 μm; ( C ) the expression level of transcription factors in CD4 + T cells. n = 5/group, data are shown with mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001 vs. CIA group by one-way ANOVA test, ns indicates no significant difference).

Article Snippet: After 30 min of incubation at 4 °C in the dark, cells were washed with PBS, followed by the addition of Transcription Factor Fix/Perm working solution (TONBO, USA) and incubation at 4 °C for 1 h. After the Transcription Factor Perm working solution (TONBO, USA) was washed off, the cells were stained with intracellular/nuclear antibodies, namely anti-IFN-γ-violetFluor450 (Clone: XMG1.2, TONBO, USA), anti-IL-4-PE (Clone: 11B11, TONBO, USA), anti-IL-17A-PE-Cy7 (eBio17B7, eBioscience, USA), and anti-Foxp3-APC (Clone: 3G3, TONBO, USA), and incubated at 4 °C in the dark for 30 min.

Techniques: Concentration Assay, Expressing